Genomic Medicine

Engineered miniature CRISPR-Cas system for mammalian genome regulation and editing

September, 2021

In brief:

Xu et. al developed a miniature CRISPR system for genome engineering via protein and guide RNA engineering. Whereas the natural Cas12f does not function in mammalian cells, engineered Cas12f mutants, named CasMINI, show comparable activities with Cas12a for efficient gene activation. CasMINI also enables robust gene editing and base editing.